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Molecule Parameter List for PLA2-Ca*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PLA2-Ca* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
mkp1_feedback_ effects	4	Network	Shared_Object_mkp1_feedback_effects,  Sos,  PKC,   MAPK,  PLA2,  Ras,   PDGFR
This is a network involving the MAPK-PKC feedback loop with input from the PDGFR in the synapse. The distinctive feature of this model is that it includes MKP-1 induction by MAPK, and the consequent inhibitory regulation of MAPK and the feedback loop. Lots of interesting dynamics arise from this. This link provides supplementary material for the paper Bhalla US et al. Science (2002) 297(5583):1018-23. In the form of several example simulations and demos for the figures in the paper.

PLA2-Ca* acting as a Molecule in  mkp1_feedback_effects Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLA2-Ca*	mkp1_feedback_ effects Accession No. : 4	PLA2 Pathway No. : 36	0	1000	No
The generic Ca-activated form ofPLA2. Leslie and Channon 1990 BBA 1045:261.

PLA2-Ca* acting as an Enzyme in  mkp1_feedback_effects Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PLA2-Ca* / kenz	mkp1_feedback_ effects Accession No. : 4	PLA2 Pathway No. : 36	20	5.4	4	explicit E-S complex	Substrate APC Product AA
Based on Leslie and Channon 1990 BBA 1045:261, in relation to the other PLA2 inputs (not including MAPK). Ca alone is rather a weak input.

PLA2-Ca* acting as a Substrate in a reaction in  mkp1_feedback_effects Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	PIP2-Ca-PLA2-act	mkp1_feedback_ effects Accession No. : 4	PLA2 Pathway No. : 36	0.012 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 8.3333(uM)	-	Substrate PLA2-Ca* temp-PIP2 Product PIP2-Ca-PLA2*
	Synergistic activation of PLA2 by Ca and PIP2. Again from Leslie and Channon 1990 BBA 1045:261
2	DAG-Ca-PLA2-act	mkp1_feedback_ effects Accession No. : 4	PLA2 Pathway No. : 36	0.003 (uM^-1 s^-1)	4 (s^-1)	Kd(bf) = 1333.3333(uM)	-	Substrate DAG PLA2-Ca* Product DAG-Ca-PLA2*
	Synergistic activation of PLA2 by Ca and DAG. Based on Leslie and Channon 1990 BBA 1045:261 The Kd is rather large and may reflect the complications in measuring DAG. For this model it is not critical since DAG is held fixed.

PLA2-Ca* acting as a Product in a reaction in  mkp1_feedback_effects Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
PLA2-Ca-act	mkp1_feedback_ effects Accession No. : 4	PLA2 Pathway No. : 36	1 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate Ca PLA2-cytosolic Product PLA2-Ca*
Direct activation of PLA2 by Ca. From Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.