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Molecule Parameter List for E0*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

E0* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	1	0	0	2

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_Asthagiri_ adapt_fb	45	Pathway	MAPK
This is a complex model to examine mechanisms that govern MAPK pathway dynamics in Chinese hamster ovary (CHO) cell lines, particularly the role of adapter targeted negative feedback mechanism in generating complete signal adaptation. This model simulates the results as per the figure 7A of the paper by Asthagiri AR and Lauffenburger DA. Biotechnol Prog. (2001) 17(2):227-39.

E0* acting as a Molecule in  MAPK_Asthagiri_adapt_fb Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
E0*	MAPK_Asthagiri_ adapt_fb Accession No. : 45	MAPK Pathway No. : 193	0	390	No
Active form of enzyme from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39

E0* acting as an Enzyme in  MAPK_Asthagiri_adapt_fb Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
E0* / E1_activ	MAPK_Asthagiri_ adapt_fb Accession No. : 45	MAPK Pathway No. : 193	0.0600001	0.1	5	explicit E-S complex	Substrate E1 Product E1*
k1+ =6*10^8 M-1 min-1, k1- =30 min-1, kcat,1 =6 min-1 and km caluculated to 0.06 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39

E0* acting as a Substrate for an Enzyme in  MAPK_Asthagiri_adapt_fb Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
E4*  / E4*_adpt_fb	MAPK_Asthagiri_ adapt_fb Accession No. : 45	MAPK Pathway No. : 193	0.0600001	0.1	5	explicit E-S complex	Substrate E0* Product A2-
Implementation of adapter feedback induces adaptation of not only the target signal e0* but also signals downstream of the target. k5+ =6*10^8 M-1 min-1, k5- =30 min-1, kcat,5 =6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39

E0* acting as a Product in a reaction in  MAPK_Asthagiri_adapt_fb Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	C*A1-bind-A2	MAPK_Asthagiri_ adapt_fb Accession No. : 45	MAPK Pathway No. : 193	5.0001 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate A2 C*A1 Product E0*
	kf2 and kr2 are 3*10^8 M-1 min-1 and 0.5/sec from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
2	C*-bind-A1A2	MAPK_Asthagiri_ adapt_fb Accession No. : 45	MAPK Pathway No. : 193	5.0001 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate A1A2 C* Product E0*
	Kf12 and Kr12 are 3*10^8 M-1 min-1 and 0.5/sec from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39