| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
kenz
Enzyme Molecule:
PLA2-Ca* | 20 | 5.4 | 4 | explicit E-S complex | APC
| AA
|
| | Based on Leslie and Channon 1990 BBA 1045:261, in relation to the other PLA2 inputs (not including MAPK). Ca alone is rather a weak input. |
| 2 |
Enzyme Activity:
kenz
Enzyme Molecule:
PIP2-PLA2* | 20 | 11.04 | 4 | explicit E-S complex | APC
| AA
|
| | Based on Leslie and Channon 1990 BBA 1045:261. |
| 3 |
Enzyme Activity:
kenz
Enzyme Molecule:
PIP2-Ca-PLA2* | 20 | 36 | 4 | explicit E-S complex | APC
| AA
|
| | Based on AA generation by different stimuli according to Leslie and Channon 1990 BBA 1045:261 |
| 4 |
Enzyme Activity:
kenz
Enzyme Molecule:
DAG-Ca-PLA2* | 20 | 60 | 4 | explicit E-S complex | APC
| AA
|
| | Based on Leslie and Channon 1990 BBA 1045:261. |
| 5 |
Enzyme Activity:
kenz
Enzyme Molecule:
PLA2*-Ca | 20 | 120 | 4 | explicit E-S complex | APC
| AA
|
| | This form should be 3 to 6 times as fast as the Ca-only form, from Lin et al 1993 Cell 269-278 Nemenoff et al 1993 JBC 268:1960 Several forms contribute to the Ca-stimulated form, so this rate has to be a factor larger than their total contribution. I assign Vmax as the scale factor here because there is lots of APC substrate, so all the PLA2 complex enzymes are limited primarily by Vmax. |
