| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
auton_286
Enzyme Molecule:
tot_autonomous_
CaMKII | 0.00000416667 | 0.5 | 4 | explicit E-S complex | CaMKII-CaM
| CaMKII-thr286*-C
aM
|
| | The autonomous rate has a slightly higher Km than the CaM-bound rate, but Vmax is the same. Hanson and Schulman 1992 Ann Rev Biochem 61:559-601 and Hanson and Schulman 1992 JBC 267(24):17216-17224 |
| 2 |
Enzyme Activity:
auton_305
Enzyme Molecule:
tot_autonomous_
CaMKII | 0.00000416667 | 6 | 4 | explicit E-S complex | CaMKII-thr286
| CaMKII***
|
| | See Hanson and Schulman 1992 JBC 267(24):17216-17224 for afterburst rates of phosphorylation |
| 3 |
Enzyme Activity:
CaM_act_286
Enzyme Molecule:
tot_CaM_CaMKII | 0.00000270563 | 0.5 | 4 | explicit E-S complex | CaMKII-CaM
| CaMKII-thr286*-C
aM
|
| | See Hanson and Schulman 1992 JBC 267(24):17216-17224 |
| 4 |
Enzyme Activity:
CaM_act_305
Enzyme Molecule:
tot_CaM_CaMKII | 0.00000270563 | 6 | 4 | explicit E-S complex | CaMKII-thr286
| CaMKII***
|
| | Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. See especially Fig 5. |
