| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | EGF | 0 | 1000 | Yes |
| | Epidermal growth factor. |
| 2 | EGFR | 0.1667 | 1000 | No |
| | Berkers et al JBC 266 say 22K hi aff recs. Sherrill and Kyte Biochemistry 35 use range 4-200 nM. These match pretty well. Using 167 nM. |
| 3 | Internal_L.EGFR | 0 | 1000 | No |
| | The internalized and inactive ligand-bound EGFR. There is lots of interesting cell biology which we are ignoring here. |
| 4 | L.EGFR | 0 | 1000 | No |
| | This is terribly simplified: there are many interesting intermediate stages, including dimerization and assoc with adapter molecules like Shc, that contribute to the activation of the EGFR. |
| 5 | SHC | 0.5 | 1000 | No |
| | There are 2 isoforms: 52 KDa and 46 KDa (See Okada et al JBC 270:35 pp 20737 1995). They are acted up on by the EGFR in very similar ways, and apparently both bind Grb2 similarly, so we'll bundle them together here. Sasaoka et al JBC 269:51 pp 32621 1994 show immunoprecs where it looks like there is at least as much Shc as Grb2. So we'll tentatively say there is 0.5 uM of Shc. |
| 6 | SHC* | 0 | 1000 | No |
| | Phosphorylated form of SHC. Binds to the Sos.Grb2 complex to give the activated GEF form upstream of Ras. |
