| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | CaM | 20 | 1000 | No |
| | There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al, Mol Biol. of the Cell, Garland Publishers) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels. |
| 2 | neurogranin | 10 | 1000 | No |
| | Also known as RC3 and p17 and BICKS. Concentration in brain >> 2 uM from Martzen and Slemmon J Neurosci 64 92-100 1995. Concentration in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding. |
| 3 | neurogranin* | 0 | 1000 | No |
| | The phosphorylated form of neurogranin does not bind CaM (Huang et al ABB 305:2 570-580 1993) |
| 4 | neurogranin-CaM | 0 | 1000 | No |
| | This is the CaM-bound form of neurogranin. This CaM is taken out of circulation and thus reduces the signaling through CaM. |
