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Molecule Parameter List for IP3(145)

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
IP3(145) participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1105533

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Osc_Ca_
    IP3metabolism
  • 32Network
    MIPP CaMKII CaM 
    PKC IP3-3K Gq 
    PLCbeta 134_dephos 145_dephos 
    IP4-system IHP-system 1345_dephos 
    CaRegulation Othmer-Tang-model 
    This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It is similar to the Osc_Ca_IP3metab model (accession 24) except that some enzymes in the InsP3 metabolism network have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. The modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

    IP3(145) acting as a Molecule in  
    Osc_Ca_IP3metabolism Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    IP3(145)
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • IP3-3K
    Pathway No. : 162
    0.21000No
    Inositol (145)trisphosphate

    IP3(145) acting as a Summed Molecule in  
    Osc_Ca_IP3metabolism Network
    Accession NamePathway NameTargetInput
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • Othmer-Tang-mode
    l

    Pathway No. : 171
  • IP3IP3(145)
    Inositol(145)trisphosphate in the cytoplasm. Levels are determined by the IP3-metabolism network

    IP3(145) acting as a Substrate for an Enzyme in  
    Osc_Ca_IP3metabolism Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1IP3_3K  /
    ip3-3k
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • IP3-3K
    Pathway No. : 162
    1.400013.04754explicit E-S complexSubstrate
    IP3(145)

    Product
    IP4(1345)
        from Johanson et al, JBC 263; 1988 Original Vmax scaled up by 50% to obtain value at 37C, as enzyme assay was done at 30C Km increased from 0.2 to 1.4 as per various other reports (Shears Review, BiochemJ 260; 1989)
    2IP3_3K*1  /
    ip3-3k*1
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • IP3-3K
    Pathway No. : 162
    1.400040.761874.00003explicit E-S complexSubstrate
    IP3(145)

    Product
    IP4(1345)
        from Sim et al, JBC 265; 1990
    3IP3_3K*  /
    ip3-3k*
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • IP3-3K
    Pathway No. : 162
    1.400013.04754explicit E-S complexSubstrate
    IP3(145)

    Product
    IP4(1345)
        from Communi et al, EMBO J 16; 1997 In absence of CaM binding, activity same as that of non-phosphorylated enzyme
    4IP3_5pase2  /
    ip3_5pase2
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • 145_dephos
    Pathway No. : 166
    18.000410.6673.99991explicit E-S complexSubstrate
    IP3(145)

    Product
    IP2(14)
        from Hansen et al, JBC 262, 1987 activity determined from pH curve. Value scaled 1.5 times to obtain activity at 37C as original experiment was performed at 30C
    5IP_5pase1  /
    ip3_5pase1
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • 145_dephos
    Pathway No. : 166
    3.000011.14explicit E-S complexSubstrate
    IP3(145)

    Product
    IP2(14)
        Ins(145)P3 5-phosphatase activity of InsP-5-phosphatase from Hansen et al, JBC 262; 1987

    IP3(145) acting as a Product of an Enzyme in  
    Osc_Ca_IP3metabolism Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1PLC-Gq  /
    PLC-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    5754explicit E-S complexSubstrate
    PIP2

    Product
    DAG
    IP3(145)
        from Smrcka et al, 1991 Science 251: 804-807
    2PLC-Ca  /
    PLC-Ca
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    19.8408104explicit E-S complexSubstrate
    PIP2

    Product
    DAG
    IP3(145)
        From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. k1 = 1.5e-5, now 4.2e-6 k2 = 70/sec; now 40/sec k3 = 17.5/sec; now 10/sec Note that the wording in Sternweis et al is ambiguous re the Km. Also Smrcka et al; Science 251, 15.2.1991, pp804-807
    3PLC-Ca-Gq  /
    PLCb-Ca-Gq
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    4.999941604explicit E-S complexSubstrate
    PIP2

    Product
    DAG
    IP3(145)
        Km: Sternweis et al, Phil Trans R Soc Lond 1992 Vmax: Smrcka et al, Science 1991
    4PLC  /
    PLC
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    19.99942.54explicit E-S complexSubstrate
    PIP2

    Product
    DAG
    IP3(145)
        Smrcka et al; Science 251, 15.2.1991, pp804-807
    51345_3pase  /
    ip4_3pase
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • 1345_dephos
    Pathway No. : 169
    0.4000070.00124explicit E-S complexSubstrate
    IP4(1345)

    Product
    IP3(145)
        Ins(1345)P4 3-phosphatase from Nogimori et al, JBC 266; 1991

    IP3(145) acting as a Substrate in a reaction in  
    Osc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    13k-CaM*-on
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • IP3-3K
    Pathway No. : 162
    80.358
    (uM^-1 s^-1)
    180
    (s^-1)
    Kd(bf) = 2.24(uM)-Substrate
    IP3(145)
    IP3_3K_CaM*

    Product
  • 3kCaM*_ip3_
    cmplx

  •   Rates from Km of enzyme Communi et al, EMBO J 16(8)
    23k-CaM-on
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • IP3-3K
    Pathway No. : 162
    18.7497
    (uM^-1 s^-1)
    42.001
    (s^-1)
    Kd(bf) = 2.2401(uM)-Substrate
    IP3(145)
    IP3_3K_CaM

    Product
    3kCaM_ip3_cmplx
      rates from Km for enzyme: Erneux et al, Biochem 214; 1993 Enzyme is 2-2.5 fold more active than ip3-3k, but Km is doubled.
    3145-inhib-3pase
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • 1345_dephos
    Pathway No. : 169
    1
    (uM^-1 s^-1)
    1.75
    (s^-1)
    Kd(bf) = 1.75(uM)-Substrate
    1345_3pase
    IP3(145)

    Product
  • IP3(145)-3pase-c
    mplx

  •   from Hoer et al, Biochem J 270; 1990

    IP3(145) acting as a Product in a reaction in  
    Osc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1ip3(145)_trp
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • MIPP
    Pathway No. : 158
    1
    (s^-1)
    0.001
    (s^-1)
    Not applicable**-Substrate
    IP3(145)_ER

    Product
    IP3(145)
      Ins(145)P3 ER-cytosol transport
    2basal
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • PLCbeta
    Pathway No. : 164
    0.003
    (s^-1)
    0
    (uM^-1 s^-1)
    --Substrate
    PIP2

    Product
    DAG
    IP3(145)
      accounts for other PLC isoforms that contribute to basal levels of IP3
    3ip4-6pase
  • Osc_Ca_
    IP3metabolism

    Accession No. : 32
  • IP4-system
    Pathway No. : 167
    0.013
    (s^-1)
    0
    (s^-1)
    --Substrate
    IP4(1456)

    Product
    IP3(145)
      this step is essential to maintain flux around the network, rate adjusted accordingly



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