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Enzyme Molecule /
Enzyme Activity | Accession Name / Accession No. | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents |
1 | Tot-active-CaMKI I / phosph_T286 | CaMKII
Accession No. 33 | CaMKII
Pathway No. 174 | 0.254651 | 10 | 0 | explicit E-S complex | Substrate: CaMKII-CaMCa4 Product : Trapped-T286
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| This enzyme represents an intra-holoenzyme reaction from one active subunit to its neighbor. k1 is now in units of 1/sec. The Km, Ratio and Vmax terms are pretty meaningless. The enzyme really is there just to represent the forward rate of 0.5 from CaMKII-CaMCa4 to Trapped-T285. We have a high k3 so that the reac is limited only by k1. The enzyme itself does not have much purpose other than to allow scaling of the overall rate by the proportion of adjacent molecules in the appropriate state. |
2 | Tot-active-CaMKI I / phosph_T305 | CaMKII
Accession No. 33 | CaMKII
Pathway No. 174 | 1.27325 | 10 | 0 | explicit E-S complex | Substrate: Autonomous Product : Capped
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| This enzyme also represents an intra-holoenzyme reaction. So it is not really treated as an enzyme in Holme's model, more like a probability of reaction that scales according to the number of active CaMKII neighboring subunits. Here we approximate it as an enzyme rate limited by the k1=0.1 step, no back reaction, and with a scaling from Total_CaMKII such that the effective forward rate will simply be the fraction of CaMKII that are active, times 1/10 for nearest neigbors, times k1. |
3 | tot_CaM_CaMKII / CaM_act_305 | fig3_CaMKII
Accession No. 2 | CaMKII
Pathway No. 13 | 0.00000270563 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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4 | tot_CaM_CaMKII / CaM_act_286 | fig3_CaMKII
Accession No. 2 | CaMKII
Pathway No. 13 | 0.00000270563 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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5 | tot_autonomous_ CaMKII / auton_305 | fig3_CaMKII
Accession No. 2 | CaMKII
Pathway No. 13 | 0.00000416667 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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6 | tot_autonomous_ CaMKII / auton_286 | fig3_CaMKII
Accession No. 2 | CaMKII
Pathway No. 13 | 0.00000416667 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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7 | tot_CaM_CaMKII / CaM_act_305 | fig4_synapse
Accession No. 3 | CaMKII
Pathway No. 26 | 0.00000270563 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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8 | tot_CaM_CaMKII / CaM_act_286 | fig4_synapse
Accession No. 3 | CaMKII
Pathway No. 26 | 0.00000270563 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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9 | tot_autonomous_ CaMKII / auton_305 | fig4_synapse
Accession No. 3 | CaMKII
Pathway No. 26 | 0.00000416667 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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10 | tot_autonomous_ CaMKII / auton_286 | fig4_synapse
Accession No. 3 | CaMKII
Pathway No. 26 | 0.00000416667 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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11 | tot_CaM_CaMKII / CaM_act_305 | Synaptic_ Network Accession No. 16 | CaMKII
Pathway No. 80 | 0.00000270563 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. See especially Fig 5. |
12 | tot_CaM_CaMKII / CaM_act_286 | Synaptic_ Network Accession No. 16 | CaMKII
Pathway No. 80 | 0.00000270563 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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| See Hanson and Schulman 1992 JBC 267(24):17216-17224 |
13 | tot_autonomous_ CaMKII / auton_305 | Synaptic_ Network Accession No. 16 | CaMKII
Pathway No. 80 | 0.00000416667 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| See Hanson and Schulman 1992 JBC 267(24):17216-17224 for afterburst rates of phosphorylation |
14 | tot_autonomous_ CaMKII / auton_286 | Synaptic_ Network Accession No. 16 | CaMKII
Pathway No. 80 | 0.00000416667 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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| The autonomous rate has a slightly higher Km than the CaM-bound rate, but Vmax is the same. Hanson and Schulman 1992 Ann Rev Biochem 61:559-601 and Hanson and Schulman 1992 JBC 267(24):17216-17224 |
15 | CaMKIIalpha / kenz | NOS_Phosph_ regulation Accession No. 20 | NOS
Pathway No. 90 | 5 | 28.5 | 4 | explicit E-S complex | Substrate: nNOS Product : NOS*
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| Hayashi et al., 1999, JBC,274(29):20597-20602. and from various other literature datas. |
16 | tot_CaM_CaMKII / CaM_act_305 | NonOsc_Ca_ IP3metabolism Accession No. 23 | CaMKII
Pathway No. 106 | 0.00000270563 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. See especially Fig 5. |
17 | tot_CaM_CaMKII / CaM_act_286 | NonOsc_Ca_ IP3metabolism Accession No. 23 | CaMKII
Pathway No. 106 | 0.00000270563 | 0.5 | 4 | explicit E-S complex | Substrate: CaMKII-CaM Product : CaMKII-thr286*-C aM
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| See Hanson and Schulman 1992 JBC 267(24):17216-17224 |
18 | tot_CaM_CaMKII / CaM-CaMK-phos | NonOsc_Ca_ IP3metabolism Accession No. 23 | CaMKII
Pathway No. 106 | 1.60001 | 0.5 | 4 | explicit E-S complex | Substrate: IP3_3K Product : IP3_3K*
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| rates referred from standard CaM-CaMKII phosphorylation rates |
19 | tot_CaM_CaMKII / CaM-CaMK-phos1 | NonOsc_Ca_ IP3metabolism Accession No. 23 | CaMKII
Pathway No. 106 | 1.59998 | 0.5 | 4 | explicit E-S complex | Substrate: IP3_3K_CaM Product : IP3_3K_CaM*
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| rates referred from standard CaM-CaMKII phosphorylation rates |
20 | tot_autonomous_ CaMKII / auton_305 | NonOsc_Ca_ IP3metabolism Accession No. 23 | CaMKII
Pathway No. 106 | 0.00000416667 | 6 | 4 | explicit E-S complex | Substrate: CaMKII-thr286 Product : CaMKII***
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| See Hanson and Schulman 1992 JBC 267(24):17216-17224 for afterburst rates of phosphorylation |