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Molecule Parameter List for Ca.PLCbeta

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

Ca.PLCbeta participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
PLCbeta1	13	Pathway	PLCbeta
This is a model of PLCbeta1 in the bovine brain membranes as stimulated by Ca, Gq and PA. The primary data sources are Irene Litosch Biochemistry (2000) 39(26):7736-43, Sternweis PC et al. Philos Trans R Soc Lond B Biol Sci. (1992) 336(1276):35-41 and Smrcka AV et al. Science (1991) 251(4995):804-7.

Ca.PLCbeta acting as a Molecule in  PLCbeta1 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Ca.PLCbeta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	0	0.0016667	No
6 Nov 2001 The enzyme activity is observed only when it combines with Ca and becomes Calcium bound form of enzyme complex.All the curves obtained are in the presence of different concentrations of Calcium by I. Litosch, Biochem.,2000, 39,7736-7743.

Ca.PLCbeta acting as an Enzyme in  PLCbeta1 Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
Ca.PLCbeta / Ca.PLCbeta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	10	7	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate PIP2 Product DAG IP3
3 Nov 2001 Sternweis et al Phil Trans Soc R Lond B Biol Sci 1992 Apr 29; 336(1276);35-41;discussion 41-2. The Km and Vmax values according to Sternweis et al. The Km is fixed to 10uM since there is absence of Gq and so is the Vmax values which is fixed at 7uM since the Km obtained varies between 5 and 20uM and Vmax between 7 and 23umol/min/mg depending on the presence and absence of Gq.

Ca.PLCbeta acting as a Substrate in a reaction in  PLCbeta1 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Gq-bind-Ca.PLCbe ta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	50 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.02(uM)	-	Substrate Ca.PLCbeta Gq Product Gq.Ca.PLCbeta
	6 Nov 2001 The Kf and Kb values were fixed according to the response curves obtained from I. Litosch, Biochem., 2000, 39, 7736-7743.
2	PA-bind-Ca.PLCbe ta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	1 (uM^-1 s^-1)	8 (s^-1)	Kd(bf) = 8(uM)	-	Substrate Ca.PLCbeta PA Product PA.Ca.PLCbeta
	6 Nov 2001 The Kf and Kb values were fixed according to the dose response curves obtained from I. Litosch, Biochem., 2000, 39, 7736-7743.

Ca.PLCbeta acting as a Product in a reaction in  PLCbeta1 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Ca_bind_PLCbeta	PLCbeta1 Accession No. : 13	PLCbeta Pathway No. : 67	1 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 1(uM)	-	Substrate Ca PLCbeta Product Ca.PLCbeta
6 Nov 2001 The Kf and Kb values were fixed according to the dose response curves obtained from I.Litosch, Biochem., 2000, 39, 7736-7743.