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Result: 1 - 20 of 29 rows are displayed Previous of 2  Next

Reaction List for Accession fig3_CaMKII (Accession Number 2)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name		Pathway Name / 
Pathway No.		KfKbKdtauReagents
1 Ca-bind-CaNABPP2B

Pathway No. 16		10008
(uM^-2 s^-1)		1
(s^-1)		Kd(af) = 0.01(uM)-  Substrate:
 CaNAB
 Ca
 Ca

 Products:
 CaNAB-Ca2
2 
  • CaMK-thr286-bind
    -CaM
    		• CaMKII

    Pathway No. 13    		1000.2
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0001(uM)-  Substrate:
     CaMKII-thr286
     CaM-Ca4

     Products:
  •  CaMKII-thr286*-C
    aM
    • 3 Gs-bind-AC2*AC

    Pathway No. 18    		833.28
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0012(uM)-  Substrate:
     Gs-alpha
     AC2*

     Products:
     AC2*-Gs
    4 CaM_bind_PDE1AC

    Pathway No. 18    		720
    (uM^-1 s^-1)    		5
    (s^-1)    		Kd(bf) = 0.0069(uM)-  Substrate:
     PDE1
     CaM-Ca4

     Products:
     CaM.PDE1
    5 
  • CaMCa4-bind-CaNA
    B
    		• PP2B

    Pathway No. 16    		600
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0017(uM)-  Substrate:
     CaM-Ca4
     CaNAB-Ca4

     Products:
     CaMCa4-CaNAB
    6 Gs-bind-AC2AC

    Pathway No. 18    		499.998
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.002(uM)-  Substrate:
     AC2
     Gs-alpha

     Products:
     AC2-Gs
    7 Inact-PP1PP1

    Pathway No. 15    		499.98
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0002(uM)-  Substrate:
     I1*
     PP1-active

     Products:
     PP1-I1*
    8 Gs-bind-AC1AC

    Pathway No. 18    		126
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0079(uM)-  Substrate:
     Gs-alpha
     AC1

     Products:
     AC1-Gs
    9 
  • cAMP-bind-site-A
    1
    		• PKA

    Pathway No. 17    		75
    (uM^-1 s^-1)    		110
    (s^-1)    		Kd(bf) = 1.4667(uM)-  Substrate:
     R2C2-cAMP2
     cAMP

     Products:
     R2C2-cAMP3
     Kf = 75 /sec/uM, Kb = 110 /sec; This site has higher Kd for cAMP (kinetics within bovine myocardium)
    Dagfinn Ogreid and Stein Ove Doskeland (1981) FEBS Lett. 129(2):287-292
    10 
  • cAMP-bind-site-A
    2
    		• PKA

    Pathway No. 17    		75
    (uM^-1 s^-1)    		32.5
    (s^-1)    		Kd(bf) = 0.4333(uM)-  Substrate:
     cAMP
     R2C2-cAMP3

     Products:
     R2C2-cAMP4
     Now cAMP shows effects of cooperativity and PKA has a low Kd for cAMP.
    11 CaM-TR2-bind-CaCaM

    Pathway No. 14    		72
    (uM^-2 s^-1)    		72
    (s^-1)    		Kd(af) = 1(uM)-  Substrate:
     CaM
     Ca
     Ca

     Products:
     CaM-TR2-Ca2
    12 Release-C1PKA

    Pathway No. 17    		60
    (s^-1)    		18
    (uM^-1 s^-1)    		Kd(cb) = 0.3(uM)-  Substrate:
     R2C2-cAMP4

     Products:
     PKA-active
     R2C-cAMP4
     The complex starts to dissociate and release catalytic subunit C. This has to be fast, as the activation of PKA by cAMP is also fast.
    13 Release-C2PKA

    Pathway No. 17    		60
    (s^-1)    		18
    (uM^-1 s^-1)    		Kd(cb) = 0.3(uM)-  Substrate:
     R2C-cAMP4

     Products:
     PKA-active
     R2-cAMP4
    14 inhib-PKAPKA

    Pathway No. 17    		60
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0167(uM)-  Substrate:
     PKA-active
     PKA-inhibitor

     Products:
     inhibited-PKA
    15 
  • cAMP-bind-site-B
    1
    		• PKA

    Pathway No. 17    		54
    (uM^-1 s^-1)    		33
    (s^-1)    		Kd(bf) = 0.6111(uM)-  Substrate:
     R2C2
     cAMP

     Products:
     R2C2-cAMP
     Kf = 54 /sec/uM, Kb = 33 /sec; PKA in normal human T lymphocytes. Hasler et al (1992) FASEB J 6:2735-2741
    Kd =1e-07 M for type II, 5.6e-08 M for type I; Stephen B. Smith et al (1981) PNAS, USA 78: 1591-1595
    Ka1 = 2.1e+07 /M which gives Kd = 47 nM, Kan = 5e+08 /M or Kd of 2nM from Fig.7
    16 
  • cAMP-bind-site-B
    2
    		• PKA

    Pathway No. 17    		54
    (uM^-1 s^-1)    		33
    (s^-1)    		Kd(bf) = 0.6111(uM)-  Substrate:
     R2C2-cAMP
     cAMP

     Products:
     R2C2-cAMP2
     Kf = 54 /sec/uM, Kb = 33 /sec; Same Km (1e-07M) assumed as site B1.
    kf/kb = 0.7e-07M*1e06/(6e05^02) = 2e-13:2.77e-12
    17 CaMKII-bind-CaMCaMKII

    Pathway No. 13    		49.9998
    (uM^-1 s^-1)    		5
    (s^-1)    		Kd(bf) = 0.1(uM)-  Substrate:
     CaM-Ca4
     CaMKII

     Products:
     CaMKII-CaM
    18 CaM-bind-AC1AC

    Pathway No. 18    		49.9998
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.02(uM)-  Substrate:
     CaM-Ca4
     AC1

     Products:
     AC1-CaM
    19 
  • CaM-TR2-Ca2-bind
    -Ca
    		• CaM

    Pathway No. 14    		3.6
    (uM^-1 s^-1)    		10
    (s^-1)    		Kd(bf) = 2.7778(uM)-  Substrate:
     CaM-TR2-Ca2
     Ca

     Products:
     CaM-Ca3
    20 
  • Ca-bind-CaNAB-Ca
    2
    		• PP2B

    Pathway No. 16    		3.6
    (uM^-2 s^-1)    		1
    (s^-1)    		Kd(af) = 0.527(uM)-  Substrate:
     Ca
     Ca
     CaNAB-Ca2

     Products:
     CaNAB-Ca4
     
    Result: 1 - 20 of 29 rows are displayed Previous of 2  Next


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