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Result: 1 - 20 of 29 rows are displayed Previous of 2  Next

Reaction List for Accession fig3_CaMKII (Accession Number 2)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name		Pathway Name / 
Pathway No.		KfKbKdtauReagents
1 Release-C2PKA

Pathway No. 17		60
(s^-1)		18
(uM^-1 s^-1)		Kd(cb) = 0.3(uM)-  Substrate:
 R2C-cAMP4

 Products:
 PKA-active
 R2-cAMP4
2 Release-C1PKA

Pathway No. 17		60
(s^-1)		18
(uM^-1 s^-1)		Kd(cb) = 0.3(uM)-  Substrate:
 R2C2-cAMP4

 Products:
 PKA-active
 R2C-cAMP4
 The complex starts to dissociate and release catalytic subunit C. This has to be fast, as the activation of PKA by cAMP is also fast.
3 
  • neurogranin-bind
    -CaM
    		• CaM

    Pathway No. 14    		0.3
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 3.3333(uM)-  Substrate:
     neurogranin
     CaM

     Products:
     neurogranin-CaM
    4 inhib-PKAPKA

    Pathway No. 17    		60
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0167(uM)-  Substrate:
     PKA-active
     PKA-inhibitor

     Products:
     inhibited-PKA
    5 Inact-PP1PP1

    Pathway No. 15    		499.98
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0002(uM)-  Substrate:
     I1*
     PP1-active

     Products:
     PP1-I1*
    6 Gs-bind-AC2*AC

    Pathway No. 18    		833.28
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0012(uM)-  Substrate:
     Gs-alpha
     AC2*

     Products:
     AC2*-Gs
    7 Gs-bind-AC2AC

    Pathway No. 18    		499.998
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.002(uM)-  Substrate:
     AC2
     Gs-alpha

     Products:
     AC2-Gs
    8 Gs-bind-AC1AC

    Pathway No. 18    		126
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0079(uM)-  Substrate:
     Gs-alpha
     AC1

     Products:
     AC1-Gs
    9 dissoc-PP1-I1PP1

    Pathway No. 15    		1
    (s^-1)    		0
    (uM^-1 s^-1)    		--  Substrate:
     PP1-I1

     Products:
     PP1-active
     I1
    10 dephosph-PDEAC

    Pathway No. 18    		0.1
    (s^-1)    		0
    (s^-1)    		--  Substrate:
     cAMP-PDE*

     Products:
     cAMP-PDE
    11 
  • dephosph-neurogr
    anin
    		• CaM

    Pathway No. 14    		0.005
    (s^-1)    		0
    (s^-1)    		--  Substrate:
     neurogranin*

     Products:
     neurogranin
    12 dephosph-AC2AC

    Pathway No. 18    		0.1
    (s^-1)    		0
    (s^-1)    		--  Substrate:
     AC2*

     Products:
     AC2
    13 CaM_bind_PDE1AC

    Pathway No. 18    		720
    (uM^-1 s^-1)    		5
    (s^-1)    		Kd(bf) = 0.0069(uM)-  Substrate:
     PDE1
     CaM-Ca4

     Products:
     CaM.PDE1
    14 
  • cAMP-bind-site-B
    2
    		• PKA

    Pathway No. 17    		54
    (uM^-1 s^-1)    		33
    (s^-1)    		Kd(bf) = 0.6111(uM)-  Substrate:
     R2C2-cAMP
     cAMP

     Products:
     R2C2-cAMP2
     Kf = 54 /sec/uM, Kb = 33 /sec; Same Km (1e-07M) assumed as site B1.
    kf/kb = 0.7e-07M*1e06/(6e05^02) = 2e-13:2.77e-12
    15 
  • cAMP-bind-site-B
    1
    		• PKA

    Pathway No. 17    		54
    (uM^-1 s^-1)    		33
    (s^-1)    		Kd(bf) = 0.6111(uM)-  Substrate:
     R2C2
     cAMP

     Products:
     R2C2-cAMP
     Kf = 54 /sec/uM, Kb = 33 /sec; PKA in normal human T lymphocytes. Hasler et al (1992) FASEB J 6:2735-2741
    Kd =1e-07 M for type II, 5.6e-08 M for type I; Stephen B. Smith et al (1981) PNAS, USA 78: 1591-1595
    Ka1 = 2.1e+07 /M which gives Kd = 47 nM, Kan = 5e+08 /M or Kd of 2nM from Fig.7
    16 
  • cAMP-bind-site-A
    2
    		• PKA

    Pathway No. 17    		75
    (uM^-1 s^-1)    		32.5
    (s^-1)    		Kd(bf) = 0.4333(uM)-  Substrate:
     cAMP
     R2C2-cAMP3

     Products:
     R2C2-cAMP4
     Now cAMP shows effects of cooperativity and PKA has a low Kd for cAMP.
    17 
  • cAMP-bind-site-A
    1
    		• PKA

    Pathway No. 17    		75
    (uM^-1 s^-1)    		110
    (s^-1)    		Kd(bf) = 1.4667(uM)-  Substrate:
     R2C2-cAMP2
     cAMP

     Products:
     R2C2-cAMP3
     Kf = 75 /sec/uM, Kb = 110 /sec; This site has higher Kd for cAMP (kinetics within bovine myocardium)
    Dagfinn Ogreid and Stein Ove Doskeland (1981) FEBS Lett. 129(2):287-292
    18 CaMKII-bind-CaMCaMKII

    Pathway No. 13    		49.9998
    (uM^-1 s^-1)    		5
    (s^-1)    		Kd(bf) = 0.1(uM)-  Substrate:
     CaM-Ca4
     CaMKII

     Products:
     CaMKII-CaM
    19 
  • CaMK-thr286-bind
    -CaM
    		• CaMKII

    Pathway No. 13    		1000.2
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0001(uM)-  Substrate:
     CaMKII-thr286
     CaM-Ca4

     Products:
  •  CaMKII-thr286*-C
    aM
    • 20 
  • CaMCa4-bind-CaNA
    B
    		• PP2B

    Pathway No. 16    		600
    (uM^-1 s^-1)    		1
    (s^-1)    		Kd(bf) = 0.0017(uM)-  Substrate:
     CaM-Ca4
     CaNAB-Ca4

     Products:
     CaMCa4-CaNAB
     
    Result: 1 - 20 of 29 rows are displayed Previous of 2  Next


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