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"Connected to" List for CamKII

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Statistics

CamKII participated as Model  Pathway  Molecule  Sum total of  Enzyme  Substrate of an enzyme  Product of an enzyme  Reaction  Substrate in reaction  Product in a reaction 
No. of occurrences27342478218120920854116112

CamKII found in these Models

Model in which CamKII acting as a molecule or reaction or enzyme are listed here.
 Name /
Accession No.		Accession
Type /
Entry Date		Model TypeAccession StatisticsCamKII StatisticSpecies /
Tissue		Cell Compartment /
Source		Methodology /
Model_Implementation
6
  • Osc_Ca_
    IP3metabolism    /
    Accession No. : 24
    		• Network /
    2002-01-08 00:00:00    		ChemicalMolecule = 137
    Enzyme   = 49
    Reaction  = 82
    		Molecule = 8
    Enzyme   = 8
    Reaction  = 1
    		Generic mammalian /
    Brain - Neuronal    		Cytosol /
    Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. ( peer-reviewed publication )    		Quantitative match to experiments, Qualitative /
    Approximate implementation
        This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It differs from the NonOsc_Ca_IP3metabolism network in the CaRegulation module and in InsP3 receptor kinetics. Details of InsP3 receptor kinetics have been adapted from the Othmer-Tang model for oscillatory Ca dynamics. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.
    7
  • NonOsc_Ca_
    IP3metabolism    /
    Accession No. : 31
    		• Network /
    2002-04-03 00:00:00    		ChemicalMolecule = 135
    Enzyme   = 42
    Reaction  = 90
    		Molecule = 8
    Enzyme   = 8
    Reaction  = 1
    		Generic mammalian /
    Brain - Neuronal    		Cytosol /
    Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. ( peer-reviewed publication )    		Quantitative match to experiments, Qualitative /
    Exact GENESIS implementation
        This network models detailed metabolism of Ins(145)P3, integrated with GPCR mediated PLCbeta activation and Ca release by the InsP3 receptor in the neuron. It is similar to the NonOsc_Ca_IP3metab model (accession 23) except that some enzymes have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. These modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.
    8
  • Osc_Ca_
    IP3metabolism    /
    Accession No. : 32
    		• Network /
    2002-04-03 00:00:00    		ChemicalMolecule = 138
    Enzyme   = 43
    Reaction  = 91
    		Molecule = 8
    Enzyme   = 8
    Reaction  = 1
    		Generic mammalian /
    Brain - Neuronal    		Cytosol /
    Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. ( peer-reviewed publication )    		Quantitative match to experiments, Qualitative /
    Exact GENESIS implementation
        This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It is similar to the Osc_Ca_IP3metab model (accession 24) except that some enzymes in the InsP3 metabolism network have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. The modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.
    9CaMKII/
    Accession No. : 33    		Network /
    2002-08-21 00:00:00    		ChemicalMolecule = 23
    Enzyme   = 2
    Reaction  = 27
    		Molecule = 8
    Enzyme   = 2
    Reaction  = 9
    		Generic mammalian /
    Brain - Neuronal    		Synapse /
    William R. Holmes J Comput Neurosci. (2000) 8(1):65-85 ( peer-reviewed publication )    		Qualitative /
    Approximate implementation
        This is a deterministic, point kinetics approximation to the dendritic spine CaMKII model described in William R. Holmes J Comput Neurosci. (2000) 8(1):65-85. Rates are the same but the responses differ somewhat because this model does not include the stochastic and diffusive calculations of the original.
    10CaMKII_2003/
    Accession No. : 49    		Network /
    2003-04-28 00:00:00    		ChemicalMolecule = 31
    Enzyme   = 18
    Reaction  = 15
    		Molecule = 7
    Enzyme   = 4
    Reaction  = 1
    		Generic mammalian /
    Brain - Neuronal    		Synapse /
    Bhalla US. Biophys J. 2004 Aug;87(2):733-44. ( peer-reviewed publication ).    		Qualitative /
    Exact GENESIS implementation
        Model of regulation of CaMKII by Calcium, including parallel excitatory input from CaM and inhibitory input from PP1 as regulated by Calcineurin and PKA. Cell type: neuronal.
    Bhalla US. Biophys J. 2004 Aug;87(2):733-44.
    Statistics To see the complete set of data click here. To see Previous or Next set of entry click here

    CamKII found in these Pathways

    Pathway in which CamKII acting as a molecule or reaction or enzyme are listed here.
     Pathway
    Name    		Accession
    Name    		Model Type /
    Accession Type    		Pathway StatisticsCamKII StatisticsSource /
    Entry
    Date
    6CaMKII

    Pathway No. : 13    		fig3_CaMKII

    Accession No. : 2    		Chemical /
    Network    		Molecule = 9
    Enzyme   = 4
    Reaction  = 3
    		Molecule = 8
    Enzyme   = 4
    Reaction  = 1
    		Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. ( peer-reviewed publication ) /
    2001-11-07 00:00:00
       Related Pathway :
    26, 80, 106, 121, 145, 159, 174, 202, 216, 235, 245, 258, 264, 272, 282, 322, 339, 357
        This is the model file for figure 3 from Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. It is a model of the Ca activation of CaMKII and other CaM-activated enzymes. It includes the regulatory phosphatases PP1 and PP2B (Calcineurin) acting on CaMKII and also includes CaM-activated adenylyl cyclase and PKA in the synapse.
    Demonstration script files for generating the figures in the paper, including figure 3, are available here.
    7
  • Shared_Object_
    fig3_CaMKII
    Pathway No. : 12
    		• fig3_CaMKII

    Accession No. : 2    		Chemical /
    Network    		Molecule = 15
    Enzyme   = 16
    Reaction  = 0
    		Molecule = 1
    Enzyme   = 0
    Reaction  = 0
    		Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. ( peer-reviewed publication ) /
    2001-11-07 00:00:00
       Related Pathway :
        This is the model file for figure 3 from Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. It is a model of the Ca activation of CaMKII and other CaM-activated enzymes. It includes the regulatory phosphatases PP1 and PP2B (Calcineurin) acting on CaMKII and also includes CaM-activated adenylyl cyclase and PKA in the synapse.
    Demonstration script files for generating the figures in the paper, including figure 3, are available here.
    8CaMKII

    Pathway No. : 26    		fig4_synapse

    Accession No. : 3    		Chemical /
    Network    		Molecule = 9
    Enzyme   = 4
    Reaction  = 3
    		Molecule = 8
    Enzyme   = 4
    Reaction  = 1
    		Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. ( peer-reviewed publication ) /
    2001-11-07 00:00:00
       Related Pathway :
    13, 80, 106, 121, 145, 159, 174, 202, 216, 235, 245, 258, 264, 272, 282, 322, 339, 357
        This is the composite model of 4 kinases: PKC, MAPK, PKA and CaMKII and numerous regulatory pathways involved in synaptic signaling. From Bhalla US and Iyengar R. Science (1999) 283(5400):381-7.This model comes from figure 4 of that paper.
    Demonstration script files for generating the figures in the paper, including figure 4, are available here.
    9
  • Shared_Object_
    fig4_synapse
    Pathway No. : 19
    		• fig4_synapse

    Accession No. : 3    		Chemical /
    Network    		Molecule = 28
    Enzyme   = 28
    Reaction  = 2
    		Molecule = 1
    Enzyme   = 0
    Reaction  = 0
    		Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. ( peer-reviewed publication ) /
    2001-11-07 00:00:00
       Related Pathway :
        This is the composite model of 4 kinases: PKC, MAPK, PKA and CaMKII and numerous regulatory pathways involved in synaptic signaling. From Bhalla US and Iyengar R. Science (1999) 283(5400):381-7.This model comes from figure 4 of that paper.
    Demonstration script files for generating the figures in the paper, including figure 4, are available here.
    10CaMKII

    Pathway No. : 80
  • Synaptic_
    Network
    Accession No. : 16
    		• Chemical /
    Network    		Molecule = 9
    Enzyme   = 4
    Reaction  = 3
    		Molecule = 8
    Enzyme   = 4
    Reaction  = 1
    		Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. ( peer-reviewed publication ) /
    2001-12-12 00:00:00
       Related Pathway :
    13, 26, 106, 121, 145, 159, 174, 202, 216, 235, 245, 258, 264, 272, 282, 322, 339, 357
        This model is an annotated version of the synaptic signaling network.
    The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated.
    Bhalla US Biophys J. 2002 Aug;83(2):740-52
    Bhalla US J Comput Neurosci. 2002 Jul-Aug;13(1):49-62
    Statistics To see the complete set of data click here. To see Previous or Next set of entry click here

    CamKII acting as a Molecule

     NameAccession Name
    Accession No.    		Pathway Name
    Pathway No.    		Initial Conc.(uM)Volume(fL)Buffered
    6actCaMKII-PSD
  • AMPAR_traff_
    model1
    Accession No. 60
    		• CaMKII

    Pathway No. 245    		20.01No
    7actCaMKII-PSD
  • CaMKII_noPKA_
    model3
    Accession No. 62
  • Shared_Object_
    CaMKII_noPKA_
    model3

    Pathway No. 257
    		• 20.01No
    8actCaMKII-PSDCaMKII_model3
    Accession No. 63
  • Shared_Object_
    CaMKII_model3

    Pathway No. 263
    		• 20.01No
    9actCaMKII-PSD
  • AMPAR_CaMKII_
    strong_coupling
    Accession No. 64
  • Shared_Object_
    AMPAR_CaMKII_
    strong_coupling

    Pathway No. 271
    		• 20.01No
    10actCaMKII-PSD
  • AMPAR_CaMKII_
    weak_coupling
    Accession No. 65
    		• CaMKII_PSD

    Pathway No. 292    		20.01No
    Statistics To see the complete set of data click here. To see Previous or Next set of entry click here

    CamKII acting as Summed Molecule

     Accession Name
    Accession No.    		Pathway Name
    Pathway No.    		TargetInput
    6fig4_synapse
    Accession No. 3    		CaMKII
    Pathway No. 26
  • tot_autonomous_
    CaMKII
    		• CaMKII-thr286
    CaMKII***
    7
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		tot_CaM_CaMKII
    		CaMKII-CaM
  • CaMKII-thr286*-C
    aM
    •     This pool sums the levels of the CaM-bound forms of CaMKII: CaMKII-CaM + CaMKII-thr286*-CaM. Although their phosphorylation states are different, the level of activity is about the same so it makes sense to sum the levels. Hanson et al 1994 Neuron 12:943-956
    8
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80
  • tot_autonomous_
    CaMKII
    		• CaMKII-thr286
    CaMKII***
        This is the sum total of the various CaM-independent forms of the kinase. There are actually several possible states here, but I only consider the forms thr-286 phosphorylated form and the doubly/triply phosphorylated form including the thr305/306, represented here as CaMKII***
    9
  • NonOsc_Ca_
    IP3metabolism
    Accession No. 23
    		• CaMKII
    Pathway No. 106    		tot_CaM_CaMKII
    		CaMKII-CaM
  • CaMKII-thr286*-C
    aM
    •     This pool sums the levels of the CaM-bound forms of CaMKII: CaMKII-CaM + CaMKII-thr286*-CaM. Although their phosphorylation states are different, the level of activity is about the same so it makes sense to sum the levels. Hanson et al 1994 Neuron 12:943-956
    10
  • NonOsc_Ca_
    IP3metabolism
    Accession No. 23
    		• CaMKII
    Pathway No. 106
  • tot_autonomous_
    CaMKII
    		• CaMKII-thr286
    CaMKII***
        This is the sum total of the various CaM-independent forms of the kinase. There are actually several possible states here, but I only consider the forms thr-286 phosphorylated form and the doubly/triply phosphorylated form including the thr305/306, represented here as CaMKII***
    Statistics To see the complete set of data click here. To see Previous or Next set of entry click here

    CamKII acting as an Enzyme

     Enzyme Molecule /
    Enzyme Activity    		Accession Name
    Accession No.    		Pathway Name
    Pathway No.    		Km (uM)kcat (s^-1)RatioEnzyme TypeReagents
    6tot_CaM_CaMKII
      /
    CaM_act_286
    		fig4_synapse
    Accession No. 3    		CaMKII
    Pathway No. 26    		0.000002705630.54explicit E-S complexSubstrate:
    CaMKII-CaM

    Product:
    CaMKII-thr286*-C
    aM
    7
  • tot_autonomous_
    CaMKII
      /
    auton_305
    		• fig4_synapse
    Accession No. 3    		CaMKII
    Pathway No. 26    		0.0000041666764explicit E-S complexSubstrate:
    CaMKII-thr286

    Product:
    CaMKII***
    8
  • tot_autonomous_
    CaMKII
      /
    auton_286
    		• fig4_synapse
    Accession No. 3    		CaMKII
    Pathway No. 26    		0.000004166670.54explicit E-S complexSubstrate:
    CaMKII-CaM

    Product:
    CaMKII-thr286*-C
    aM
    9tot_CaM_CaMKII
      /
    CaM_act_305
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		0.0000027056364explicit E-S complexSubstrate:
    CaMKII-thr286

    Product:
    CaMKII***
        Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. See especially Fig 5.
    10tot_CaM_CaMKII
      /
    CaM_act_286
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		0.000002705630.54explicit E-S complexSubstrate:
    CaMKII-CaM

    Product:
    CaMKII-thr286*-C
    aM
        See Hanson and Schulman 1992 JBC 267(24):17216-17224
    Statistics To see the complete set of data click here. To see Previous or Next set of entry click here

    CamKII acting as a Subtrate for an Enzyme

     Enzyme Molecule /
    Enzyme Activity    		Accession Name
    Accession No.    		Pathway Name
    Pathway No.    		Km (uM)kcat (s^-1)RatioEnzyme TypeReagents
    6tot_CaM_CaMKII  /
    CaM_act_286
    		fig3_CaMKII
    Accession No. 2    		CaMKII
    Pathway No. 13    		0.000002705630.54explicit E-S complexSubstrate:
    CaMKII-CaM

    Product:
  • CaMKII-thr286*-C
    aM
    • 7tot_autonomous_
    CaMKII      /
    auton_305
    		fig3_CaMKII
    Accession No. 2    		CaMKII
    Pathway No. 13    		0.0000041666764explicit E-S complexSubstrate:
    CaMKII-thr286

    Product:
    CaMKII***
    8tot_autonomous_
    CaMKII      /
    auton_286
    		fig3_CaMKII
    Accession No. 2    		CaMKII
    Pathway No. 13    		0.000004166670.54explicit E-S complexSubstrate:
    CaMKII-CaM

    Product:
  • CaMKII-thr286*-C
    aM
    • 9PP1-active  /
    Deph-thr286
    		fig4_synapse
    Accession No. 3
  • Shared_Object_
    fig4_synapse
    Pathway No. 19
    		• 5.099070.354explicit E-S complexSubstrate:
  • CaMKII-thr286*-C
    aM

    Product:
    CaMKII-CaM
    • 10PP1-active  /
    Deph-thr305
    		fig4_synapse
    Accession No. 3
  • Shared_Object_
    fig4_synapse
    Pathway No. 19
    		• 5.099070.354explicit E-S complexSubstrate:
    CaMKII***

    Product:
    CaMKII-thr286
    Statistics To see the complete set of data click here. To see Previous or Next set of entry click here

    CamKII acting as a Product for an Enzyme

     Enzyme Molecule /
    Enzyme Activity    		Accession Name
    Accession No.    		Pathway Name
    Pathway No.    		Km (uM)kcat (s^-1)RatioEnzyme TypeReagents
    6tot_CaM_CaMKII  /
    CaM_act_286
    		fig3_CaMKII
    Accession No. 2    		CaMKII
    Pathway No. 13    		0.000002705630.54explicit E-S complexSubstrate:
    CaMKII-CaM

    Product:
  • CaMKII-thr286*-C
    aM
    • 7tot_autonomous_
    CaMKII      /
    auton_305
    		fig3_CaMKII
    Accession No. 2    		CaMKII
    Pathway No. 13    		0.0000041666764explicit E-S complexSubstrate:
    CaMKII-thr286

    Product:
    CaMKII***
    8tot_autonomous_
    CaMKII      /
    auton_286
    		fig3_CaMKII
    Accession No. 2    		CaMKII
    Pathway No. 13    		0.000004166670.54explicit E-S complexSubstrate:
    CaMKII-CaM

    Product:
  • CaMKII-thr286*-C
    aM
    • 9PP1-active  /
    Deph-thr286
    		fig4_synapse
    Accession No. 3
  • Shared_Object_
    fig4_synapse
    Pathway No. 19
    		• 5.099070.354explicit E-S complexSubstrate:
  • CaMKII-thr286*-C
    aM

    Product:
    CaMKII-CaM
    • 10PP1-active  /
    Deph-thr305
    		fig4_synapse
    Accession No. 3
  • Shared_Object_
    fig4_synapse
    Pathway No. 19
    		• 5.099070.354explicit E-S complexSubstrate:
    CaMKII***

    Product:
    CaMKII-thr286
    Statistics To see the complete set of data click here. To see Previous or Next set of entry click here

    CamKII acting as a Reaction

    Keq is calculated only for first order reactions. Kd is calculated only for second order reactions. [ nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
     NameAccession Name
    Accession No.    		Pathway Name
    Pathway No.    		KfKbKdtauReagents
    6 CaMKII-bind-CaM
  • NonOsc_Ca_
    IP3metabolism
    Accession No. 31
    		• CaMKII
    Pathway No. 145    		49.9998
    uM^-1 s^-1    		5
    s^-1    		Kd(bf) = 0.1(uM)-Substrate:
    CaM-Ca4
    CaMKII

    Product:
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kd = 0.1 uM. Rate is fast (see Hanson et al Neuron 12 943-956 1994) Hanson and Schulman 1992 AnnRev Biochem 61:559-601 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    7 CaMKII-bind-CaM
  • Osc_Ca_
    IP3metabolism
    Accession No. 32
    		• CaMKII
    Pathway No. 159    		49.9998
    uM^-1 s^-1    		5
    s^-1    		Kd(bf) = 0.1(uM)-Substrate:
    CaM-Ca4
    CaMKII

    Product:
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kd = 0.1 uM. Rate is fast (see Hanson et al Neuron 12 943-956 1994) Hanson and Schulman 1992 AnnRev Biochem 61:559-601 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    8 CaMKII-bind-CaMCaMKII
    Accession No. 33    		CaMKII
    Pathway No. 174    		0.2
    uM^-1 s^-1    		2700
    s^-1    		Kd(bf) = 13500(uM)-Substrate:
    CaM
    CaMKII

    Product:
    CaMKII-CaM
    9 CaMKII-bind-CaMCaMKII_2003
    Accession No. 49    		CaMKII
    Pathway No. 202    		49.9998
    uM^-1 s^-1    		5
    s^-1    		Kd(bf) = 0.1(uM)-Substrate:
    CaM-Ca4
    CaMKII

    Product:
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kb/Kf = 6e4 #/cell. Rate is fast (see Hanson et al Neuron 12 943-956 1994) so lets say kb = 10. This gives kf = 1.6667e-4 H&S AnnRev Biochem 92 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    10 CaMKII-bind-CaM
  • MAPK_network_
    2003
    Accession No. 50
    		• CaMKII
    Pathway No. 216    		49.9998
    uM^-1 s^-1    		5
    s^-1    		Kd(bf) = 0.1(uM)-Substrate:
    CaM-Ca4
    CaMKII

    Product:
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kb/Kf = 6e4 #/cell. Rate is fast (see Hanson et al Neuron 12 943-956 1994) so lets say kb = 10. This gives kf = 1.6667e-4 H&S AnnRev Biochem 92 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    Statistics To see the complete set of data Click here To see Previous or Next set of entry click here

    CamKII acting as a Substrate in a Reaction

    Keq is calculated only for first order reactions. Kd is calculated only for second order reactions. [ nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
     NameAccession Name
    Accession No.    		Pathway Name
    Pathway No.    		KfKbKdtauReagents
    6 basal-activityfig4_synapse
    Accession No. 3    		CaMKII
    Pathway No. 26    		0.003
    s^-1    		0
    s^-1    		--Substrate:
    CaMKII

    Product:
    CaMKII-thr286
    7 CaMKII-bind-CaM
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		49.9998
    uM^-1 s^-1    		5
    s^-1    		Kd(bf) = 0.1(uM)-Substrate:
    CaM-Ca4
    CaMKII

    Product:
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kd = 0.1 uM. Rate is fast (see Hanson et al Neuron 12 943-956 1994) Hanson and Schulman 1992 AnnRev Biochem 61:559-601 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    8
  • CaMK-thr286-bind
    -CaM
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		1000.2
    uM^-1 s^-1    		0.1
    s^-1    		Kd(bf) = 0.0001(uM)-Substrate:
    CaMKII-thr286
    CaM-Ca4

    Product:
  • CaMKII-thr286*-C
    aM
    •   Affinity is up 1000X over the unphosphorylated CaMKII, which makes the Kd of 0.1 nM. See Hanson et al 1994 Neuron 12:943-956. Time to release is about 20 sec, so the kb is OK at 0.1/sec. as tested by a few runs.
    9 basal-activity
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		0.003
    s^-1    		0
    s^-1    		--Substrate:
    CaMKII

    Product:
    CaMKII-thr286
      This reaction represents one of the unknowns in CaMK-II biochemistry: what maintains the basal level of phosphorylation on thr 286 ? See Hanson and Schulman Ann Rev Biochem 1992 61:559-601, specially pg 580, for review. I have not been able to find any compelling mechanism in the literature, but fortunately the level of basal activity is well documented. Lisman et al propose that the levels of PP1 are very low in the postsynaptic density, and PP2A is excluded from the PSD, and this would lead to autophosphorylation at a sustained level.
    10 CaMKII-bind-CaM
  • NonOsc_Ca_
    IP3metabolism
    Accession No. 23
    		• CaMKII
    Pathway No. 106    		49.9998
    uM^-1 s^-1    		5
    s^-1    		Kd(bf) = 0.1(uM)-Substrate:
    CaM-Ca4
    CaMKII

    Product:
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kd = 0.1 uM. Rate is fast (see Hanson et al Neuron 12 943-956 1994) Hanson and Schulman 1992 AnnRev Biochem 61:559-601 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    Statistics To see the complete set of data Click here To see Previous or Next set of entry click here

    CamKII acting as a Product in a Reaction

    Keq is calculated only for first order reactions. Kd is calculated only for second order reactions. [ nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
     NameAccession Name
    Accession No.    		Pathway Name
    Pathway No.    		KfKbKdtauReagents
    6 basal-activityfig4_synapse
    Accession No. 3    		CaMKII
    Pathway No. 26    		0.003
    s^-1    		0
    s^-1    		--Substrate:
    CaMKII

    Product:
    CaMKII-thr286
    7 CaMKII-bind-CaM
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		49.9998
    uM^-1 s^-1    		5
    s^-1    		Kd(bf) = 0.1(uM)-Substrate:
    CaM-Ca4
    CaMKII

    Product:
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kd = 0.1 uM. Rate is fast (see Hanson et al Neuron 12 943-956 1994) Hanson and Schulman 1992 AnnRev Biochem 61:559-601 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    8
  • CaMK-thr286-bind
    -CaM
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		1000.2
    uM^-1 s^-1    		0.1
    s^-1    		Kd(bf) = 0.0001(uM)-Substrate:
    CaMKII-thr286
    CaM-Ca4

    Product:
  • CaMKII-thr286*-C
    aM
    •   Affinity is up 1000X over the unphosphorylated CaMKII, which makes the Kd of 0.1 nM. See Hanson et al 1994 Neuron 12:943-956. Time to release is about 20 sec, so the kb is OK at 0.1/sec. as tested by a few runs.
    9 basal-activity
  • Synaptic_
    Network
    Accession No. 16
    		• CaMKII
    Pathway No. 80    		0.003
    s^-1    		0
    s^-1    		--Substrate:
    CaMKII

    Product:
    CaMKII-thr286
      This reaction represents one of the unknowns in CaMK-II biochemistry: what maintains the basal level of phosphorylation on thr 286 ? See Hanson and Schulman Ann Rev Biochem 1992 61:559-601, specially pg 580, for review. I have not been able to find any compelling mechanism in the literature, but fortunately the level of basal activity is well documented. Lisman et al propose that the levels of PP1 are very low in the postsynaptic density, and PP2A is excluded from the PSD, and this would lead to autophosphorylation at a sustained level.
    10 CaMKII-bind-CaM
  • NonOsc_Ca_
    IP3metabolism
    Accession No. 23
    		• CaMKII
    Pathway No. 106    		49.9998
    uM^-1 s^-1    		5
    s^-1    		Kd(bf) = 0.1(uM)-Substrate:
    CaM-Ca4
    CaMKII

    Product:
    CaMKII-CaM
      This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kd = 0.1 uM. Rate is fast (see Hanson et al Neuron 12 943-956 1994) Hanson and Schulman 1992 AnnRev Biochem 61:559-601 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
    Statistics To see the complete set of data Click here To see Previous or Next set of entry click here


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