|
Reaction Name | Accession Name / Accession No. | Pathway Name / Pathway No. | Kf | Kb | Kd | tau | Reagents |
21 | CaMK-thr286-bind -CaM | Synaptic_ Network Accession No. 16 | CaMKII
Pathway No. 80 | 1000.2 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X over the unphosphorylated CaMKII, which makes the Kd of 0.1 nM. See Hanson et al 1994 Neuron 12:943-956. Time to release is about 20 sec, so the kb is OK at 0.1/sec. as tested by a few runs. | 22 | CaMK-thr286-bind -CaM | NonOsc_Ca_ IP3metabolism Accession No. 23 | CaMKII
Pathway No. 106 | 1000.2 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X over the unphosphorylated CaMKII, which makes the Kd of 0.1 nM. See Hanson et al 1994 Neuron 12:943-956. Time to release is about 20 sec, so the kb is OK at 0.1/sec. as tested by a few runs. | 23 | CaMK-thr286-bind -CaM | Osc_Ca_ IP3metabolism Accession No. 24 | CaMKII
Pathway No. 121 | 1000.2 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X over the unphosphorylated CaMKII, which makes the Kd of 0.1 nM. See Hanson et al 1994 Neuron 12:943-956. Time to release is about 20 sec, so the kb is OK at 0.1/sec. as tested by a few runs. | 24 | CaMK-thr286-bind -CaM | NonOsc_Ca_ IP3metabolism Accession No. 31 | CaMKII
Pathway No. 145 | 1000.2 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X over the unphosphorylated CaMKII, which makes the Kd of 0.1 nM. See Hanson et al 1994 Neuron 12:943-956. Time to release is about 20 sec, so the kb is OK at 0.1/sec. as tested by a few runs. | 25 | CaMK-thr286-bind -CaM | Osc_Ca_ IP3metabolism Accession No. 32 | CaMKII
Pathway No. 159 | 1000.2 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X over the unphosphorylated CaMKII, which makes the Kd of 0.1 nM. See Hanson et al 1994 Neuron 12:943-956. Time to release is about 20 sec, so the kb is OK at 0.1/sec. as tested by a few runs. | 26 | CaMK-thr286-bind -CaM | CaMKII_2003
Accession No. 49 | CaMKII
Pathway No. 202 | 1000.2 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 27 | CaMK-thr286-bind -CaM | MAPK_network_ 2003 Accession No. 50 | CaMKII
Pathway No. 216 | 1000.2 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 28 | CaMK-thr286-bind -CaM | AMPAR_traff_ model0 Accession No. 59 | CaMKII
Pathway No. 235 | 1000.19 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 29 | CaMK-thr286-bind -CaM | AMPAR_traff_ model1 Accession No. 60 | CaMKII
Pathway No. 245 | 1000.19 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 30 | CaMK-thr286-bind -CaM | CaMKII_noPKA_ model3 Accession No. 62 | CaMKII
Pathway No. 258 | 1000.19 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 31 | CaMK-thr286-bind -CaM | CaMKII_model3
Accession No. 63 | CaMKII
Pathway No. 264 | 1000.19 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 32 | CaMK-thr286-bind -CaM | AMPAR_CaMKII_ strong_coupling Accession No. 64 | CaMKII
Pathway No. 272 | 1000.19 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 33 | CaMK-thr286-bind -CaM | AMPAR_CaMKII_ weak_coupling Accession No. 65 | CaMKII
Pathway No. 282 | 1000.19 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 34 | CaMK-thr286-bind -CaM | Ajay_Bhalla_ 2004_PKM_Tuning Accession No. 76 | CaMKII
Pathway No. 322 | 1000.17 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 35 | CaMK-thr286-bind -CaM | Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. 77 | CaMKII
Pathway No. 339 | 1000.17 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 36 | CaMK-thr286-bind -CaM | Ajay_Bhalla_ 2004_Feedback_ Tuning Accession No. 78 | CaMKII
Pathway No. 357 | 1000.17 (uM^-1 s^-1) | 0.1 (s^-1) | Kd(bf) = 0.0001(uM) | - | Substrate: CaMKII-thr286 CaM-Ca4
Products: CaMKII-thr286*-C aM
|
| Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3 | 37 | CaMKII-bind-CaM | fig3_CaMKII
Accession No. 2 | CaMKII
Pathway No. 13 | 49.9998 (uM^-1 s^-1) | 5 (s^-1) | Kd(bf) = 0.1(uM) | - | Substrate: CaM-Ca4 CaMKII
Products: CaMKII-CaM
|
38 | CaMKII-bind-CaM | fig4_synapse
Accession No. 3 | CaMKII
Pathway No. 26 | 49.9998 (uM^-1 s^-1) | 5 (s^-1) | Kd(bf) = 0.1(uM) | - | Substrate: CaM-Ca4 CaMKII
Products: CaMKII-CaM
|
39 | CaMKII-bind-CaM | Synaptic_ Network Accession No. 16 | CaMKII
Pathway No. 80 | 49.9998 (uM^-1 s^-1) | 5 (s^-1) | Kd(bf) = 0.1(uM) | - | Substrate: CaM-Ca4 CaMKII
Products: CaMKII-CaM
|
| This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kd = 0.1 uM. Rate is fast (see Hanson et al Neuron 12 943-956 1994) Hanson and Schulman 1992 AnnRev Biochem 61:559-601 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol. | 40 | CaMKII-bind-CaM | NonOsc_Ca_ IP3metabolism Accession No. 23 | CaMKII
Pathway No. 106 | 49.9998 (uM^-1 s^-1) | 5 (s^-1) | Kd(bf) = 0.1(uM) | - | Substrate: CaM-Ca4 CaMKII
Products: CaMKII-CaM
|
| This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kd = 0.1 uM. Rate is fast (see Hanson et al Neuron 12 943-956 1994) Hanson and Schulman 1992 AnnRev Biochem 61:559-601 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol. |